The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute Cell Cycle Assay, a selection of Apoptosis Assays and the standard Cell Count and Viability Assay..
With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView™ software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.
This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.
The disposable Via1-Cassette™ this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.
Read more about the Cassette technology
| Application | Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, and DNA fragmentation assays. User-adapted protocols with the FlexiCyte™ module |
| Cell type(s) | Mammalian, yeast, insect, avian |
| Software | NucleoView™ |
| Consumable(s) | Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™ |
| Capacity (Analysis time) |
Variable, depending on consumable and assay type |
| Loading volume | 60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™) |
| Analysis volume | 3.2 μl sample (Via1-Cassette™), 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™) |
| Optimal range | 5×104 to 5×106 cells/ml |
| Staining dye(s) | Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined |
| Excitation | Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs |
| Emission (nm) | Nine interchangeable emission filters: 430/20, 470/55
475/15, 530/15, 560/35, 580/25, 675/75, 630 LP, 740/60 |
| Optics | Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels |
| Data output | Images, tables, histograms, scatter plots |
| Data export formats | .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs |
| Product Number | 970-3002 (Fixed Analytical Assays); 970-3003 (FlexiCyte™) |
| Size | 29 x 29 x 31 cm (W x H x D) |
| Weight | 14 kg (30.9 lb) |
| Supply voltage | 24VDC (100–240 V ~ 50–60 Hz) |
