{"id":522,"date":"2024-10-18T11:33:39","date_gmt":"2024-10-18T09:33:39","guid":{"rendered":"http:\/\/gandginstruments.hu\/?p=522"},"modified":"2024-10-21T12:13:57","modified_gmt":"2024-10-21T10:13:57","slug":"chemometec-kazettas-technologia-hogyan-mukodik","status":"publish","type":"post","link":"https:\/\/gandginstruments.hu\/en\/chemometec-kazettas-technologia-hogyan-mukodik\/","title":{"rendered":"Chemometec kazett\u00e1s technol\u00f3gia – Hogyan m\u0171k\u00f6dik?"},"content":{"rendered":"
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Kazett\u00e1s technol\u00f3gia<\/h1>\nA precizit\u00e1s, pontoss\u00e1g \u00e9s reproduk\u00e1lhat\u00f3s\u00e1g kik\u00fcsz\u00f6b\u00f6li az emberi hibalehet\u0151s\u00e9geket a sejtsz\u00e1ml\u00e1l\u00e1s sor\u00e1n.\n\nA t\u00e9rfogat-kalibr\u00e1lt, egyszer haszn\u00e1latos Via1\u2122<\/a> and Via2\u2122<\/a> t\u00edpus\u00fa kazett\u00e1k egyszer\u0171 megold\u00e1st k\u00edn\u00e1lnak az optim\u00e1lis sejtsz\u00e1ml\u00e1l\u00e1s<\/a> \u00e9rdek\u00e9ben. Az eldobhat\u00f3 m\u0171anyag kazett\u00e1k tartalmazz\u00e1k a m\u00e9r\u00e9shez haszn\u00e1latos fluoreszcens fest\u00e9keket. A sejtmint\u00e1t \u00fagy t\u00f6ltheti a kazett\u00e1kba, hogy a be\u00e9p\u00edtett pipett\u00e1t egyszer\u0171en belem\u00e1rtja a sejtszuszpenzi\u00f3ba, \u00e9s megnyomja a dugatty\u00fat.\n

Hogyan m\u0171k\u00f6dik?<\/h2>\n\"\"<\/strong>\n

Integr\u00e1lt pipetta<\/strong><\/h2>\nA Via1-Cassette\u2122 \u00e9s a Via2-Cassette\u2122 egyszer haszn\u00e1latos pipettak\u00e9nt m\u0171k\u00f6dik, el\u0151re bet\u00f6lt\u00f6tt fest\u00e9kekkel, leegyszer\u0171s\u00edtve a sejtminta kinyer\u00e9s\u00e9t, a sejtek fest\u00e9kekkel val\u00f3 kever\u00e9s\u00e9t \u00e9s a minta bet\u00f6lt\u00e9s\u00e9t a sejtsz\u00e1ml\u00e1l\u00f3ba, mindezt a kezel\u0151i torz\u00edt\u00e1s kik\u00fcsz\u00f6b\u00f6l\u00e9se \u00e9rdek\u00e9ben. A dugatty\u00fa megnyom\u00e1sa v\u00e1kuumot hoz l\u00e9tre, amely a szok\u00e1sos pipett\u00e1z\u00e1si hib\u00e1k n\u00e9lk\u00fcl h\u00fazza be a sejtmint\u00e1t a sejtfest\u0151 csatorn\u00e1kba, ez\u00e1ltal n\u00f6velve a pontoss\u00e1got.\n\nA kazett\u00e1kat immobiliz\u00e1lt akridin-naranccsal (AO) \u00e9s 4′,6-diamidino-2-fenilindollal (DAPI) t\u00f6lt\u00f6tt\u00e9k meg a teljes sejt-, illetve az elhalt sejtpopul\u00e1ci\u00f3k jel\u00f6l\u00e9s\u00e9re. A m\u0171szer szoftvere minden egyes alkalommal k\u00f6vetkezetesen elemzi az AO \u00e9s a DAPI fluoreszcencia adatokat. Az emberi hib\u00e1z\u00e1s \u00edgy nem befoly\u00e1solja a sejtek meghat\u00e1roz\u00e1s\u00e1t \u00e9s az elemz\u00e9si eredm\u00e9nyeket.\n\nAz eredm\u00e9nyek tov\u00e1bbi pontoss\u00e1ga \u00e9s reproduk\u00e1lhat\u00f3s\u00e1ga \u00e9rdek\u00e9ben a kazetta m\u00e9r\u0151kamr\u00e1ja el\u0151re kalibr\u00e1lt t\u00e9rfogattal rendelkezik, amelyet a m\u0171szer minden egyes mintaelemz\u00e9s sor\u00e1n k\u00fcl\u00f6n-k\u00fcl\u00f6n \u00e9rz\u00e9kel. A felhaszn\u00e1l\u00f3 vagy a pipett\u00e1z\u00e1s hib\u00e1i nem zavarj\u00e1k a m\u00e9rt adatokat, \u00e9s a m\u0171szer megmondja, hogy a felhaszn\u00e1l\u00f3 az aj\u00e1nlott sejtkoncentr\u00e1ci -tartom\u00e1nyon bel\u00fcl dolgozik-e.\n

Kever\u0151 csatorn\u00e1k<\/h2>\nA Via1-Cassette\u2122 \u00e9s Via2-Cassette\u2122 kever\u0151csatorn\u00e1k el\u0151ret\u00f6ltve vannak AO-val a teljes sejtfest\u00e9shez \u00e9s DAPI-val a nem \u00e9letk\u00e9pes sejtek fest\u00e9s\u00e9hez. A kever\u0151csatorn\u00e1k bonyolult alakja biztos\u00edtja a sejtek \u00e9s a fest\u00e9kek egyenletes kevered\u00e9s\u00e9t, \u00e9s a m\u00e9r\u0151kamr\u00e1ba homog\u00e9n minta ker\u00fcl.\n

Kazettakalibr\u00e1l\u00e1s – a pontk\u00f3drendszer<\/h2>\nA Via1-Cassette\u2122 \u00e9s a Via2-Cassette\u2122 kazett\u00e1k k\u00fcl\u00f6n-k\u00fcl\u00f6n t\u00e9rfogatkalibr\u00e1ltak \u00e9s pontk\u00f3ddal vannak ell\u00e1tva, hogy a m\u00e9r\u0151kamra t\u00e9rfogata minden kazett\u00e1ban pontos legyen. Egy szabv\u00e1nyos hemocitom\u00e9terben a t\u00e1vols\u00e1g ak\u00e1r 10-50%-kal is v\u00e1ltozhat, ami pontatlan sejtkoncentr\u00e1ci\u00f3 sz\u00e1m\u00edt\u00e1sokhoz vezet.\n

T\u00e9rfogat-kalibr\u00e1lt m\u00e9r\u0151kamra<\/h2>\nA NucleoCounter\u00ae NC-200\u2122, <\/a>NC-202\u2122<\/a> and NC-3000\u2122<\/a> m\u0171szerek \u00e1ltal elemzett teljes t\u00e9rfogat ak\u00e1r 28-szorosa is lehet egy k\u00e9zi sejtsz\u00e1ml\u00e1l\u00f3 kamra egy n\u00e9gyzet\u00e9ben megsz\u00e1ml\u00e1lt t\u00e9rfogatnak, ez\u00e1ltal jav\u00edtva a statisztikai pontoss\u00e1got.\n\nKazetta \u00f6sszehasonl\u00edt\u00e1s<\/strong>\n

\"\"<\/h1>\n

Biztons\u00e1gos, nem toxikus sejtsz\u00e1ml\u00e1l\u00f3 rendszer<\/h2>\nSz\u00e1mos sejtsz\u00e1ml\u00e1l\u00f3 m\u00f3dszer, legyen az automatiz\u00e1lt vagy k\u00e9zi, trypan-k\u00e9ket haszn\u00e1l az elhalt sejtek fest\u00e9s\u00e9re \u00e9s azonos\u00edt\u00e1s\u00e1ra.The <\/a>trypan-k\u00e9k m\u00e9rgez\u0151<\/a>, a 2B csoportba tartoz\u00f3 r\u00e1kkelt\u0151 anyagk\u00e9nt van besorolva, \u00e9s a labor\u00e1ns szem\u00e9lyzetet a fest\u00e9k \u00e9s a sejtminta pipett\u00e1z\u00e1sa \u00e9s kezel\u00e9se sor\u00e1n k\u00e1ros expoz\u00edci\u00f3 vesz\u00e9ly\u00e9nek teszi ki. A kazett\u00e1k el\u0151ret\u00f6ltve vannak AO-val \u00e9s DAPI-val az \u00f6sszes sejt, illetve az elhalt sejtek fest\u00e9s\u00e9hez.\n\nEmellett a kazett\u00e1k egyedi gy\u00e1rt\u00e1s\u00fa, egyszer haszn\u00e1latos m\u0171anyag eszk\u00f6z\u00f6k, amelyeket az optim\u00e1lis mintakezel\u00e9s \u00e9s a biztons\u00e1gos \u00e1rtalmatlan\u00edt\u00e1s \u00e9rdek\u00e9ben terveztek. A minta \u00e9s a fest\u00e9kek a kazett\u00e1ban vannak, \u00e9s haszn\u00e1lat k\u00f6zben nem szabadulnak ki. Haszn\u00e1lat ut\u00e1n biztons\u00e1gosan eldobhat\u00f3, t\u00e1mogatva a biztons\u00e1gos \u00e9s tiszta munkak\u00f6rnyezetet.\n

Propidium-jodid alap\u00fa kazett\u00e1k a reproduk\u00e1lhat\u00f3 sejtsz\u00e1ml\u00e1l\u00e1shoz<\/h2>\nA NucleoCassette\u2122, <\/a>PI-Cassette\u2122, <\/a>SP1-Cassette\u2122<\/a> and SCC-Cassette\u2122<\/a> a NucleoCounter\u00ae 100\u2122 sorozat\u00fa automatiz\u00e1lt sejtsz\u00e1ml\u00e1l\u00f3<\/u> \u00e9s a NucleoCounter\u00ae NC-3000\u2122 sejtanaliz\u00e1tor fejlett k\u00e9pi citom\u00e9ter k\u00eds\u00e9r\u0151 kazett\u00e1i.\n\nEzek a kazett\u00e1k propidium-jodidot (PI) tartalmaznak, a sejtmagokhoz k\u00f6t\u0151d\u0151 fluorofort, amely a NucleoView\u2122 <\/a>szoftverrel<\/a> v\u00e9gzett k\u00e9pelemz\u00e9s sor\u00e1n piros sz\u00ednnel kiemelkedik.<\/a>\n\nA Via1-Cassette\u2122 \u00e9s a Via2-Cassette\u2122 k\u00e9sz\u00fcl\u00e9khez hasonl\u00f3an a kazett\u00e1k csatorn\u00e1i immobiliz\u00e1lt PI-t tartalmaznak, ahol a sejtminta \u00e9s a reagens keveredik az elemz\u00e9s el\u0151tt.\n\nA z\u00e1rt kazetta kialak\u00edt\u00e1s\u00e1val a felhaszn\u00e1l\u00f3 elker\u00fcli a fest\u00e9kkel \u00e9s a mint\u00e1val val\u00f3 \u00e9rintkez\u00e9st, ami biztos\u00edtja a felhaszn\u00e1l\u00f3 biztons\u00e1g\u00e1t \u00e9s kik\u00fcsz\u00f6b\u00f6li az emberi beavatkoz\u00e1s lehet\u0151s\u00e9g\u00e9t.\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

Szeretn\u00e9 meg\u00e9rteni hogyan m\u0171k\u00f6dnek a Chemometec sejtsz\u00e1ml\u00e1l\u00f3 k\u00e9sz\u00fcl\u00e9keinek haszn\u00e1lat\u00e1hoz sz\u00fcks\u00e9ges kazett\u00e1k? Tudjon meg t\u00f6bbet lenti bejegyz\u00e9s\u00fcnkb\u0151l!<\/p>","protected":false},"author":6,"featured_media":3698,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[1],"tags":[135,134],"class_list":["post-522","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized","tag-chemometec","tag-sejtszamlalas"],"acf":[],"yoast_head":"\nChemometec kazett\u00e1s technol\u00f3gia - Hogyan m\u0171k\u00f6dik? - G&G<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/gandginstruments.hu\/en\/chemometec-kazettas-technologia-hogyan-mukodik\/\" \/>\n<meta property=\"og:locale\" content=\"en_GB\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Chemometec kazett\u00e1s technol\u00f3gia - Hogyan m\u0171k\u00f6dik? - G&G\" \/>\n<meta property=\"og:description\" content=\"Szeretn\u00e9 meg\u00e9rteni hogyan m\u0171k\u00f6dnek a Chemometec sejtsz\u00e1ml\u00e1l\u00f3 k\u00e9sz\u00fcl\u00e9keinek haszn\u00e1lat\u00e1hoz sz\u00fcks\u00e9ges kazett\u00e1k? 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